aka The 378-Dimensional Individual Olfactory Receptor Subtype Genome
aka The Olfactory Fingerprint
I would like to go back to Conspiracy Keanu on this one,
because I know you feel the same way.
It's pretty hard to tell if the Blue you see is the same
Blue I see. (And The Dress
is still fresh enough in our collective memory to know how this plays out.)
For the most part, we can be sure that when we're
detecting energy in the 610-670 THz range, it will be called Blue by anyone who
knows what Blue means (which is not everyone; check this out for a lesson on
the cultural
evolution of color terms).
Smells however, not the same. Isovaleric Acid is the best
example of this, because half the people who smell it will call it delicious
and the other half disgusting. So we have differences in opinion, big deal. But
there's more – we are not perceiving the same thing.
That is to say, the hundreds of chemically-sensitive
receptors in our nose, which are programmed by hundreds of genes in our
personal genome, they're pretty personal. And they can be quite different from
person to person, with as high as a 30% variation. This
means the actual receptors encoding molecular features into our minds are not
all the same. This isn't just about subjectivity; the actual hardware is
different from person to person.
General olfactory acuity will change based on age,
gender, smoking habits, body type, and race. Other factors such as prior upper
respiratory infection, trauma, and environmental toxin exposure are also
involved. Even something as simple as prior exposure to an
odor can change the way we perceive it (in the case of androstenone). Not only
that, the same odor will smell different to the same person at different times!
With all that variation, how do we ever really know what
each other is talking about? My androstenone is not your androstenone. A great
study from a powerhouse in olfactory research was done back in 2012 to
characterize these perceptual differences across a diverse metropolitan
population, and I'm summarizing it here because without it, all other smell
research is kind of useless.
The Study
They gave 66 different odors to 391 people who closely
reflected the diverse population of New York City (meaning that this study
group has a fighting chance of representing the diversity of the human genome).
The Findings
·
Young, female, non-smoking subjects had the
highest average olfactory acuity.
·
Deviations from normal body type in either
direction were associated with decreased olfactory acuity.
·
General olfactory acuity declines with age.
·
Reduced general olfactory acuity can be caused
by genetics, trauma, exposure to toxic agents, neurodegenerative diseases, or
infections.
Differences in Olfactory Acuity Between Races
·
African-Americans 149
·
Asians 231
·
Caucasians 225
· (Asians
are more similar to each other, and African-Americans are more different)
On Hedonics, i.e., Pleasant vs Unpleasant
·
Unpleasant odours were generally perceived to be
more intense than pleasant odours
·
Across all subjects, the eight most pleasant
odours were food odours such as vanilla, citrus, minty, and cinnamon odours
·
The seven least pleasant odours were fatty acid
derivatives associated with the sour smell of rancid butter or body odour
·
The biggest variability in pleasantness
perception was found for floral odours
·
The two most pleasant stimuli were the two
concentrations of ethyl vanillin, followed by the high concentration of
vanillin
·
The least liked odor was isovaleric acid (rancid
butter) and isobutyraldehyde (sour)
·
Throughout our subject population, odours
perceived to be most and least pleasant were remarkably stable
·
For 18 of the 134 stimuli the pleasantness
rating differed significantly between African-American and Caucasian subjects
·
The biggest difference between younger and older
subjects was that older subjects perceived anise, the odour of liquorice, to be
more pleasant
*I thought this one was easy,
since I know that most candy experience by someone born in ~1930 came from
licorice, and that changes pretty drastically thereafter; the first Twizzler flavor was
licorice, not strawberry, which didn't appear until the late 1970's.
·
Men vs Women: there are older studies that show
differences, but these were not reproduced in this study, instead, new sets:
For guaiacol, the odour of wood smoke, both concentrations were perceived to be
significantly more pleasant by men. The high concentration of guaiacol showed
the largest difference between men and women
·
All the odours that were perceived to be more
pleasant by perfume users were odours used in perfumes (pentadecalactone,
heptyl acetate, octyl aldehyde, nonyl aldehyde); Perfume use may result in
these odours being rated as more pleasant, or, alternatively, those who
perceive these odours to be more pleasant are more likely to use perfumes.
·
Geranyl acetate (floral, rose, lavender) was
perceived to be more pleasant by Caucasians than by Asians
·
Androstenone was more likely to smell “musky”
and “aromatic” to women, whereas men found it to be more “chemical” and
“sickening,”
Further Findings
Interestingly, for any given stimulus, the responses were
as similar when the ratings were spaced over one year apart as when they were
around 30 minutes apart.
This may seem surprising, but for thresholds it has even
been reported that the variability within a day is significantly larger than
the variability between days [1]. Day-to-day variability in olfactory
perception is therefore largely a consequence of sniff-to-sniff variability.
The main causes of within-individual variability are
processes that operate on the scale of seconds or minutes such as changes in
the stimulus signal-to-noise ratio [2] or the reallocation of attention by the
subject [3], rather than on the scale of hours or days, such as hormonal
changes or infections of the upper respiratory tract.
1.
Stevens JC, Cain WS, Burke RJ. Variability of olfactory thresholds. Chem
Senses. 1988;13:643–653. doi: 10.1093/chemse/13.4.643.
2.
Cain WS. Differential sensitivity for smell: “noise” at the nose. Science.
1977;195:796–798. doi: 10.1126/science.836592.
3.
Keller A. Attention and olfactory consciousness. Front Psychol. 2011;2:380.
Demography
·
Males have been shown to be more sensitive to
the odour bourgeonal (lily-of-the-valley); also the only known odour that men
are more sensitive to than women.
Olsson
P, Laska M. Human male superiority in olfactory sensitivity to the sperm
attractant odorant bourgeonal. Chem Senses. 2010;35:427–432. doi:
10.1093/chemse/bjq030.
·
African-Americans have been shown to have a
higher threshold for isovaleric acid than Caucasians, but a lower threshold for
pentadecalactone.
Whissell-Buechy
D, Amoore JE. Odour-blindness to musk: simple recessive inheritance. Nature.
1973;242:271–273. doi: 10.1038/242271a0.
·
Asians perceive each odour of the homologous
series of nonyl aldehyde, decyl aldehyde, and undecanal to be stronger than
Caucasians (no mechanistic explanation for this).
·
The three stimuli showing the greatest
variability between subjects in intensity ratings were the high concentrations
of androstenone and androstadienone, and methanethiol (cabbage-like odour
present in urine of people who have previously ingested asparagus).
Specific Odorants – Androstenone and Androstadienone
·
Altered by genetic variation in the odorous
steroid-sensitive odorant receptor OR7D4.
Keller
A, Zhuang H, Chi Q, Vosshall LB, Matsunami H. Genetic variation in a human odorant
receptor alters odour perception. Nature. 2007;449:468–472. doi:
10.1038/nature06162.
Lunde
K, Egelandsdal B, Skuterud E, Mainland JD, Lea T, Hersleth M, Matsunami H.
Genetic variation of an odorant receptor OR7D4 and sensory perception of cooked
meat containing androstenone. PLoS One. 2012;7:e35259. doi:
10.1371/journal.pone.0035259.
Knaapila
A, Zhu G, Medland SE, Wysocki CJ, Montgomery GW, Martin NG, Wright MJ, Reed DR.
A genome-wide study on the perception of the odorants androstenone and galaxolide.
Chem Senses. 2012;37:541–552. doi: 10.1093/chemse/bjs008.
·
Androstadienone was perceived to be stronger by
older subjects, women and African-Americans.
·
The functional RT variant of OR7D4 is more
common in African-Americans.
·
This is consistent with the finding from the
National Geographic Smell Survey.
Specific Odorants – Methanethiol
Associated with a single nucleotide polymorphism within a
50-gene cluster of olfactory receptors.
Pelchat
ML, Bykowski C, Duke FF, Reed DR. Excretion and perception of a characteristic
odor in urine after asparagus ingestion: a psychophysical and genetic study.
Chem Senses. 2011;36:9–17. doi: 10.1093/chemse/bjq081.
Tung
JY, Do CB, Hinds DA, Kiefer AK, Macpherson JM, Chowdry AB, Francke U, Naughton
BT, Mountain JL, Wojcicki A. et al.Efficient replication of over 180 genetic
associations with self-reported medical data. PLoS One. 2011;6:e23473. doi:
10.1371/journal.pone.0023473.
Eriksson
N, Macpherson JM, Tung JY, Hon LS, Naughton B, Saxonov S, Avey L, Wojcicki A,
Pe’er I, Mountain J. Web-based, participant-driven studies yield novel genetic
associations for common traits. PLoS Genet. 2010;6:e1000993. doi: 10.1371/journal.pgen.1000993.
Specific Odorants – Pentadecalactone, Vanillin and
Isovaleric Acid
·
Perceived as more intense by non-perfume users.
Notes
Any two individuals differ by ∼30% of their
olfactory receptor subtype genome:
Genetic variation across the human olfactory receptor repertoire alters odor perception. C. Trimmer, A. Keller, N. R. Murphy, L. L. Snyder, J. R. Willer, M. H. Nagai, N. Katsanis, L. B. Vosshall, H. Matsunami, and J. D. Mainland. PNAS p9475-9480 v116 n19. https://doi.org/10.1073/pnas.1804106115. https://www.pnas.org/doi/abs/10.1073/pnas.1804106115
Genetic variation in a human odorant receptor alters odour perception. Keller A, Zhuang H, Chi Q, Vosshall LB, Matsunami H. Nature. 2007 Sep 27;449(7161):468-72. doi: 10.1038/nature06162. Epub 2007 Sep 16. PMID: 17873857. https://pubmed.ncbi.nlm.nih.gov/17873857/
The human olfactory genome contains 418 intact odorant
receptor genes and their 912,912 intact odorant receptor alleles:
The
1000 Genomes Project (2008-2015), the largest public catalogue of human
variation and genotype data.
378-dimensional individual olfactory receptor subtype
genome:
Individual
olfactory perception reveals meaningful nonolfactory genetic information.
Secundo
L, Snitz K, Weissler K, Pinchover L, Shoenfeld Y, Loewenthal R, Agmon-Levin N,
Frumin I, Bar-Zvi D, Shushan S, Sobel N. Proc Natl Acad Sci U S A. 2015 Jul 14;
112(28):8750-5.
Androstenone perception is heavily influenced by prior
exposure:
Wysocki
CJ, Dorries KM, Beauchamp GK. Ability to perceive androstenone can be acquired
by ostensibly anosmic people. Proc Natl Acad Sci U S A. 1989;86:7976–7978. doi:
10.1073/pnas.86.20.7976.
Study on inter-individual variability:
An
olfactory demography of a diverse metropolitan population.
Keller
A, Hempstead M, Gomez IA, Gilbert AN, Vosshall LB
BMC
Neurosci. 2012 Oct 10; 13():122.
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